FB2025_02 , released April 17, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Hem, Cyfip
FlyBase ID
FBig0000090895
Interaction Network
Interactions Browser links
Hem network
Cyfip network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0179141-1.coIP.WB
Reference
Bogdan et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Hem 

Kette

bait

Myc tag

Cyfip 

Sra-1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Bogdan et al., 2004)
FBrf0179141-2.Y2H
Reference
Bogdan et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Hem 

Kette

bait

fused to GAL4 DNA-binding domain

Cyfip 

Sra-1

prey

fused to GAL4 activation domain

Cyfip 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cyfip 
C-terminal region
necessary binding region
aa 1192-1291
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bogdan et al., 2004)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Bogdan et al., 2004)
FBrf0179776-1.coIP.WB
Reference
Schenck et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Cyfip 

CYFIP

bait
Hem 

Kette

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Schenck et al., 2004)
FBrf0216441-1.coIP.MS
Reference
Gautier et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Cyfip 

Sra1

bait
Hem 

Hem/Kette

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Gautier et al., 2011)
FBrf0218395-9518.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Hem 
unspecified role
Hem 
unspecified role
Hem 
unspecified role
Cyfip 
unspecified role
Cyfip 
unspecified role
Cyfip 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 65.94826

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)