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FB2025_05
,
released December 11, 2025
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
HGScore = 65.82938
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Positive control.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
N-terminally truncated; does not contain binding sites for entire CCR4-NOT complex.
Interaction in vitro; proteins produced as a recombinant fusion proteins in baculovirus and insect cell system.
Hexameric CCR4-NOT-mini complex captured as 15 pairwise interactions.
Source was live S2R+ cells; bait and prey produced from endogenous genes.
Rga was fused to a plasma membrane or ER membrane anchor. The ability of this chimera to recruit prey protein to the plasma membrane was taken as evidence of direct physical interaction.
Source was live S2R+ cells; bait and prey produced from endogenous genes.
Not1 was fused to a plasma membrane anchor. The ability of this chimera to recruit prey protein to the plasma membrane was taken as evidence of direct physical interaction.