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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000094242
Interaction Network
Interactions Browser links
Atac2 network
Gcn5 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0204126-19a.CH.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

CG10414

neutral component

CG10414

neutral component

CG10414

neutral component
Gcn5 

Gcn5

neutral component
Gcn5 

Gcn5

neutral component
Gcn5 

Gcn5

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins derived from S2 nuclear extract.

These proteins coimmunoprecipitate with Ada2a and cofractionate with each other in size exclusion chromatography as part of 1.3-1.8 MDa ATAC histone acetyltransferase complex.

TAP-tagged Ada2a was affinity purified and co-purifying proteins were analyzed by gel filtration.

FBrf0204126-45.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Atac2

bait

HA tag, FLAG tag

Gcn5 

Gcn5

prey

11 unique peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed in stably transfected cell lines.

Prey endogenous to embryonic nuclear extract.

FBrf0214172-20.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Atac2

bait
Gcn5 

Gcn5/KAT

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Tandem affinity purification of bait and identification of prey by MudPIT.

FBrf0218284-24.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Atac2

bait

FLAG tag, HA tag

Gcn5 

Gcn5

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Tagged complexes were isolated by affinity purification and separated by size exclusion chromatography before mass spectrometry.

Interact as part of the ATAC histone acetyltransferase complex.

Bait expressed in stably transfected cells.

Prey endogenous to nuclear extract.

FBrf0218395-1810.DPiM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Gcn5 
unspecified role
Gcn5 
unspecified role
Gcn5 
unspecified role
unspecified role
unspecified role
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 98.62858

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)