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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000094912
Interaction Network
Interactions Browser links
sta network
RpS21 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0107375-1.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sta 

P40

bait

GST tag

RpS21

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Prey expressed and purified from bacteria.

Bait derived from in vitro translation.

GST pull down

FBrf0107375-2.CS.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sta 

P40

neutral component
sta 

P40

neutral component
sta 

P40

neutral component

RpS21

neutral component

RpS21

neutral component

RpS21

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Proteins endogenous to embryonic extract

RpS21 and sta proteins cosediment as part of native 40S ribosomal particle, but RpS21 is not found in polysomes. This interaction is disrupted by 500mM KCl.

FBrf0221476-658.CS.MS.EM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sta 

S2

neutral component
sta 

S2

neutral component
sta 

S2

neutral component

S21e

neutral component

S21e

neutral component

S21e

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; proteins produced from endogenous genes.

The structure of the Drosophila 80S ribosome with accessory proteins solved by cryo-EM. Complex captured as 3828 pairwise interactions which do not necessarily reflect direct contact.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)