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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000095011
Interaction Network
Interactions Browser links
sqa network
Atg1 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0213047-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Atg1 

Atg1

bait

FLAG tag

sqa 

Sqa

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was HEK 293T (human) cell line transfected with tagged Atg1 and sqa constructs.

Use a kinase substrate-trapping approach in which the catalytically inactive kinase variants formed a stable interaction with their substrates (Deminoff et al., 2006, Genetics 173: 1909–1917).

Interaction detectable only with kinase-inactive form of Atg1; not detected with wild-type Atg1 protein.

FBrf0213047-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sqa 

Sqa

bait

HA tag

sqa 
Atg1 

Atg1

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sqa 
region
unspecified role
aa 1-301

\'Sqa-K\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was HEK 293T (human) cell line transfected with tagged Atg1 and sqa constructs.

Use a kinase substrate-trapping approach in which the catalytically inactive kinase variants formed a stable interaction with their substrates (Deminoff et al., 2006, Genetics 173: 1909–1917).

Interaction assayed with kinase-inactive form of Atg1.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)