caly, Asx Physical Interaction report

General Information

Interaction Type

physical association

Interacting Genes

caly, Asx

FlyBase ID

FBig0000095150

Interaction Network

Interactions Browser links

caly network
Asx network

Reported Interactions

FBrf0210760-1.coIP.MS

Reference

Scheuermann et al., 2010

Description

physical association

Assay

anti tag coimmunoprecipitation

peptide massfingerprinting

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

caly

Calypso

bait

TAP tag

Asx

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryonic nuclear extract from transgenic line carrying TAP-tagged calypso construct.

(Scheuermann et al., 2010)

FBrf0210760-2.recon.WB

Reference

Scheuermann et al., 2010

Description

physical association

Assay

comigration in gel electrophoresis

anti tag western blot

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Asx

neutral component

HA tag

Asx

neutral component

HA tag

Asx

neutral component

HA tag

Asx

caly

Calypso

neutral component

FLAG tag

caly

Calypso

neutral component

FLAG tag

caly

Calypso

neutral component

FLAG tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Asx

region

unspecified role

aa 2-337

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Analysis of complexes reconstituted from recombinant proteins.

(Scheuermann et al., 2010)

Source was recombinant proteins produced in Sf21 cells; cell lysates mixed in vitro.

(Scheuermann et al., 2010)

FBrf0240201-1.XC

Reference

Foglizzo et al., 2018

Description

physical association

Assay

x-ray crystallography

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

caly

Calypso

neutral component

caly

Calypso

neutral component

caly

Calypso

neutral component

caly

Asx

ASX

neutral component

Asx

ASX

neutral component

Asx

ASX

neutral component

Asx

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

caly

interaction domain

sufficient binding region

aa 43-404

Asx

interaction domain

sufficient binding region

aa 207-340

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Stoichiometry 2:2

(Foglizzo et al., 2018)

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Foglizzo et al., 2018)

FBrf0240201-2.CH.MW

Reference

Foglizzo et al., 2018

Description

physical association

Assay

molecular sieving

molecular weight estimation by staining

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

caly

Calypso

neutral component

caly

Calypso

neutral component

caly

Calypso

neutral component

caly

Asx

ASX

neutral component

Asx

ASX

neutral component

Asx

ASX

neutral component

Asx

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

caly

interaction domain

sufficient binding region

aa 43-404

Asx

interaction domain

sufficient binding region

aa 207-340

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Stoichiometry 2:2 , a dimer of calypso-Asx heterodimers with dimerization occurring via the calypso coiled-coil domain. Mutation of the calypso coiled-coil results in a 1:1 calypso-Asx complex.

(Foglizzo et al., 2018)

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Foglizzo et al., 2018)

FBrf0240201-3.CS.MW

Reference

Foglizzo et al., 2018

Description

physical association

Assay

cosedimentation

molecular weight

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

caly

Calypso

neutral component

caly

Calypso

neutral component

caly

Calypso

neutral component

caly

Asx

ASX

neutral component

Asx

ASX

neutral component

Asx

ASX

neutral component

Asx

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

caly

interaction domain

sufficient binding region

aa 43-404

Asx

interaction domain

sufficient binding region

aa 207-340

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

(Foglizzo et al., 2018)

FBrf0240201-4.CL.MW

Reference

Foglizzo et al., 2018

Description

physical association

Assay

protein cross-linking with a bifunctional reagent

molecular weight

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

caly

Calypso

neutral component

caly

Calypso

neutral component

caly

Calypso

neutral component

caly

Asx

ASX

neutral component

Asx

ASX

neutral component

Asx

ASX

neutral component

Asx

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

caly

interaction domain

sufficient binding region

aa 43-404

Asx

interaction domain

sufficient binding region

aa 207-340

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Mutation of the calypso coiled-coil results in loss of the 2:2 calypso-Asx complex but not the 1:1 calypso-Asx complex.

(Foglizzo et al., 2018)

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

(Foglizzo et al., 2018)

FBrf0240201-5.SAXS

Reference

Foglizzo et al., 2018

Description

physical association

Assay

x ray scattering

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

caly

Calypso

neutral component

caly

Calypso

neutral component

caly

Calypso

neutral component

caly

Asx

ASX

neutral component

Asx

ASX

neutral component

Asx

ASX

neutral component

Asx

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

caly

interaction domain

sufficient binding region

aa 43-404

Asx

interaction domain

sufficient binding region

aa 207-340

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Stoichiometry 2:2

(Foglizzo et al., 2018)

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Foglizzo et al., 2018)

FBrf0241751-1.XC

Reference

De et al., 2019

Description

physical association

Assay

x-ray crystallography

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

caly

Calypso

neutral component

caly

Calypso

neutral component

caly

Calypso

neutral component

caly

Asx

ASX

neutral component

Asx

ASX

neutral component

Asx

ASX

neutral component

Asx

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

caly

interaction domain

sufficient binding region

aa 44-306,334-404

Asx

deubiquitinase adaptor domain

sufficient binding region

aa 207-337

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; proteins produced as recombinant fusion proteins in bacterial system.

(De et al., 2019)

FBrf0254635-3.coIP.WB

Reference

Tsuboyama et al., 2022

Description

physical association

Assay

anti bait coimmunoprecipitation

western blot

Collection

Source/Stage

Cell line used

S2-unspecified

Participants

Corresponds to

Reported as

Role

Note

caly

Calypso

bait

Asx

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Tsuboyama et al., 2022)

FBrf0254635-9.CH.MW

Reference

Tsuboyama et al., 2022

Description

physical association

Assay

molecular sieving

molecular weight estimation by staining

Collection

Source/Stage

Cell line used

S2-unspecified

Participants

Corresponds to

Reported as

Role

Note

caly

Calypso

neutral component

caly

Calypso

neutral component

caly

Calypso

neutral component

Asx

neutral component

Asx

neutral component

Asx

neutral component

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Size exclusion chromatography demonstrated an interaction between sba, caly and Asx.

(Tsuboyama et al., 2022)

Source was nuclear cell extract of S2 cell line; proteins produced from endogenous genes.

(Tsuboyama et al., 2022)

External Crossreferences and Linkouts ( 1 )

Linkouts

MIST - An integrated Molecular Interaction Database

Asx,calypso

References (4)

Report Sections

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