FB2021_02, released April 13, 2021

Physical Interaction report

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General Information
Interaction Type
physical association
Interacting Genes
SmD2, SmD1
FlyBase ID
FBig0000095195
Interaction Network
Interactions Browser links
SmD2 network
SmD1 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0218721-2.CH.MW
Reference
Chari et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
SmD1 

D1

neutral component
SmD1 

D1

neutral component
SmD1 

D1

neutral component
SmD2 

D2

neutral component
SmD2 

D2

neutral component
SmD2 

D2

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Proteins co-expressed in bacteria and the heterodimer purified by gel filtration.

(Chari et al., 2008)

SmD1 and SmD2 form a heterodimer in vitro.

(Chari et al., 2008)

Proteins expressed and purified from bacteria.

(Chari et al., 2008)
FBrf0229448-13.coIP.WB
Reference
Xiong et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
SmD2 

SmD2

bait

FLAG tag

SmD1 

SmD1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Xiong et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)