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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000095221
Interaction Network
Interactions Browser links
hth network
exd network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0098877-1.PD.WB.IM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hth 

MEIS1

bait

GST tag

exd 

Exd

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

Interspecific interaction of Dmel\exd with Mmus\Meis1 (MEIS1), orthologous to Dmel\hth (inferred by curator).

FBrf0098877-2.PD.WB.IM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hth 

MEIS1

bait

GST tag

exd 

Exd

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

Interspecific interaction of Dmel\exd with Mmus\Meis1 (MEIS1), orthologous to Dmel\hth (inferred by curator).

FBrf0108134-1.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hth 

Hth

bait

GST tag

exd 

Exd

prey

His tag, [35]S label

exd 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
exd 
PBC-A domain
sufficient binding region
aa 1-143

coordinates relative to exd-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

FBrf0123181-1.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hth 

HTH

bait

GST tag

hth 
exd 

EXD

prey

[35]S label

exd 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
hth 
HM domain
sufficient binding region
aa 1-215

"HM" construct, coordinates relative to hth-PA

exd 
PBC-A domain
necessary binding region
aa 1-143

coordinates relative to exd-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

FBrf0123181-2.EMSA.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hth 

HTH

neutral component

His tag

hth 

HTH

neutral component

His tag

hth 

HTH

neutral component

His tag

exd 

Exd

neutral component

His tag

exd 

Exd

neutral component

His tag

exd 

Exd

neutral component

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion protein in bacterial system.

hth stimulates weak binding of lab DNA by exd. Alone, neither hth nor exd binds to lab DNA.

Various combinations of hth, lab and exd proteins were mixed with [32]P labeled lab DNA. DNA-protein complexes observed by EMSA were used to deduce protein-protein interactions.

FBrf0152003-6.coIP.WB
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hth 

Hth

bait
exd 

Exd

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait endogenous to embryonic extract.

Prey endogenous to embryonic extract.

FBrf0213066-3.BiFC.FM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
exd 

exd

neutral component

Venus tag, N-terminal fragment

exd 

exd

neutral component

Venus tag, N-terminal fragment

exd 

exd

neutral component

Venus tag, N-terminal fragment

hth 

hth

neutral component

Venus tag, C-terminal fragment

hth 

hth

neutral component

Venus tag, C-terminal fragment

hth 

hth

neutral component

Venus tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

Embryos were collected overnight and incubated at 4 degrees C for 28 hrs prior to BiFC detection.

FBrf0218732-7.EMSA.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
exd 

Exd

neutral component
exd 

Exd

neutral component
exd 

Exd

neutral component
hth 

Hth

neutral component
hth 

Hth

neutral component
hth 

Hth

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced by in vitro transcription/translation.

exd and hth were mixed with [32]P labeled DNA. DNA-protein complexes observed by EMSA were used to deduce protein-protein interactions.

FBrf0219230-2.EMSA.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
exd 

exd

neutral component

His tag

exd 

exd

neutral component

His tag

exd 

exd

neutral component

His tag

hth 

hth

neutral component

His tag

hth 

hth

neutral component

His tag

hth 

hth

neutral component

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion protein in bacterial system.

exd and hth form heterodimers that bind DNA. Neither binds DNA alone.

Various combinations of hth, lab and exd proteins were mixed with [32]P labeled DNA. DNA-protein complexes observed by EMSA were used to deduce protein-protein interactions.

FBrf0219427-1a.EMSA.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
exd 

Exd

neutral component

His tag

exd 

Exd

neutral component

His tag

exd 
exd 

Exd

neutral component

His tag

exd 
hth 

Hth

neutral component

His tag

hth 
hth 

Hth

neutral component

His tag

hth 

Hth

neutral component

His tag

hth 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
exd 
homeodomain
mutation disrupting interaction
aa 51

N51A

hth 
homeodomain
mutation disrupting interaction
aa 51

N51A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system;

Electrophoretic mobility shift assays (EMSA) revealed that exd/hth could bind specifically to an evolutionarily conserved 52 base pair sequence within the Act88F enhancer region, and that DNA binding required functional homeodomains for both exd and hth, indicating that these factors can form a complex on the Act88F enhancer sequence.

FBrf0221597-1.FC
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hth 

HTH

bait

YFP tag, N-terminal fragment

exd 

EXD

prey

YFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was eye disc of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

Positive control.

FBrf0227292-377.TFIN
Description
physical association
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
exd 
unspecified role
exd 
unspecified role
exd 
unspecified role
hth 
unspecified role
hth 
unspecified role
hth 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; proteins produced from transfected construct or endogenous gene.

HGScore = 26.630672

FBrf0241977-237.Y2H
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hth 

hth

bait

fused to GAL4 DNA-binding domain

exd 

exd

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

FBrf0243360-4.BiFC.FM
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hth 

Hth

bait

YFP tag, N-terminal fragment

exd 

Exd

prey

YFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was female genital discs of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

FBrf0243360-9.BiFC.FM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hth 

Hth

bait

YFP tag, N-terminal fragment

exd 

Exd

prey

YFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was wing discs of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (12)