cosedimentation, western blot
Subregions with role in interaction
Comments concerning this interaction
Bait expressed in transiently transfected cells. Prey endogenous to nuclear extract.Hers
proteins cosediment in a single fraction as a complex with a molecular mass of about 250 kDa, consistent with the sum of the molecular mass of these three factors.Hers
protein was immunoprecipitated from nuclear extracts of cells treated with MG132 protease inhibitor, then fractionated on a glycerol density gradient.