Hers, Su(var)3-9 Interaction report

General Information

Interaction Type

physical association

Interacting Genes

Hers, Su(var)3-9

FlyBase ID

FBig0000095277

Interaction Network

Interactions Browser links

Hers network

Su(var)3-9 network

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Reported Interactions

FBrf0217607-1.coIP.WB

Reference

(Ito et al., 2012)

Description

physical association

Assay

anti bait coimmunoprecipitation, western blot

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Hers protein

HERS

bait

Su(var)3-9 protein

Su(var)3-9

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Bait endogenous to nuclear extract. Prey endogenous to nuclear extract.

FBrf0217607-3.CS.WB

Reference

(Ito et al., 2012)

Description

physical association

Assay

cosedimentation, western blot

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Hers protein

HERS

bait

FLAG tag

Su(var)3-9 protein

Su(var)3-9

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Bait expressed in transiently transfected cells. Prey endogenous to nuclear extract.Hers, Su(var)3-9 and Su(var)205 proteins cosediment in a single fraction as a complex with a molecular mass of about 250 kDa, consistent with the sum of the molecular mass of these three factors.Hers protein was immunoprecipitated from nuclear extracts of cells treated with MG132 protease inhibitor, then fractionated on a glycerol density gradient.

FBrf0217607-5.PD.A

Reference

(Ito et al., 2012)

Description

physical association

Assay

pull down, autoradiography

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Su(var)3-9 protein

Su(var)3-9

bait

GST tag

Hers protein

HERS

prey

[35]S label

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Hers

Bromo adjacent homology domain

unspecified role

aa 1037-1280

necessary and sufficient

Su(var)3-9

chromodomain

unspecified role

aa 1-278

necessary: deletion abolishes interaction

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Bait expressed and purified from bacteria. Prey derived from in vitro translation. Direct interaction of Hers with Su(var)205, bon, egg and G9a was not observed in this assay. GST pull down.

References ( 0 )

No references are recorded in FlyBase at present.