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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000095277
Interaction Network
Interactions Browser links
Su(var)3-9 network
Hers network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0217607-1.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Hers 

HERS

bait

Su(var)3-9

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait endogenous to nuclear extract.

Prey endogenous to nuclear extract.

FBrf0217607-3.CS.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Hers 

HERS

bait

FLAG tag

Su(var)3-9

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed in transiently transfected cells.

Prey endogenous to nuclear extract.

Hers, Su(var)3-9 and Su(var)205 proteins cosediment in a single fraction as a complex with a molecular mass of about 250 kDa, consistent with the sum of the molecular mass of these three factors.

Hers protein was immunoprecipitated from nuclear extracts of cells treated with MG132 protease inhibitor, then fractionated on a glycerol density gradient.

FBrf0217607-5.PD.A
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Su(var)3-9

bait

GST tag

Hers 

HERS

prey

[35]S label

Hers 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Hers 
Bromo adjacent homology domain
unspecified role
aa 1037-1280

necessary and sufficient

chromodomain
unspecified role
aa 1-278

necessary: deletion abolishes interaction

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed and purified from bacteria.

Prey derived from in vitro translation.

Direct interaction of Hers with Su(var)205, bon, egg and G9a was not observed in this assay.

GST pull down.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)