Bait endogenous to nuclear extract.

(Kyba and Brock, 1998)

Prey endogenous to nuclear extract.

(Kyba and Brock, 1998)

Source was embryonic nuclear extract of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Shao et al., 1999)

ph-p, ph-d, Pc, Psc and Scm copurify as part of a polycomb repressive (PRC1) complex

(Shao et al., 1999)

Nuclear extract was fractionated on a Biorex 70 column, then used for FLAG-affinity purification of Pc containing complexes.

(Shao et al., 1999)

The BirA biotin ligase was coexpressed to biotinylate Pc bait in vivo, which was affinity purified using streptavidin beads and eluted by cleaving the TEV site between bait and tag.

(Strübbe et al., 2011)

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Strübbe et al., 2011)

The BirA biotin ligase was coexpressed to biotinylate Pc bait in vivo, which was affinity purified using streptavidin beads and eluted by cleaving the TEV site between the bait and BioTag

(Strübbe et al., 2011)

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Strübbe et al., 2011)

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lagarou et al., 2008)

ph-d exists in Pc-bound and Pc-unbound pools.

(Lagarou et al., 2008)

Nuclear extract was partially purified by ammonium sulphate precipitation and heparin affinity chromatography before use in immunoprecipitation.

(Lagarou et al., 2008)

Positive control.

(Lagarou et al., 2008)

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Fereres et al., 2014)

Positive control

(Fereres et al., 2014)

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Fereres et al., 2014)

Positive control

(Fereres et al., 2014)

Positive control.

(Gambetta and Müller, 2014)

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Gambetta and Müller, 2014)

Positive control.

(Gambetta and Müller, 2014)

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Gambetta and Müller, 2014)