Source was embryonic nuclear extract of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
ph-p,
ph-d,
Pc,
Psc and
Scm copurify as part of a polycomb repressive (PRC1) complex
Nuclear extract was fractionated on a Biorex 70 column, then used for FLAG-affinity purification of
Pc containing complexes.