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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000095378
Interaction Network
Interactions Browser links
E2f1 network
Rbf network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0087034-1.Y2H
Reference
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
E2f1 

dE2F

bait

fused to GAL4 activation domain

E2f1 
Rbf 

RBF

prey

fused to GAL4 DNA-binding domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
E2f1 
pRB-like binding
unspecified role
aa 718-805
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD.

FBrf0087034-2.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
E2f1 

dE2F

bait
Rbf 

RBF

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait endogenous to embryonic extract.

Prey endogenous to embryonic extract.

FBrf0192129-1.coIP.WB
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rbf 

Rbf1

bait
E2f1 

E2f1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Rbf did not coimmunoprecipitate with E2f1 in extract from embryos between stages 1 and 9.

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0201281-2.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rbf 

Rbf1

bait
E2f1 

dE2F1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2* cell line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0205842-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
E2f1 

E2F1

bait
Rbf 

Rbf1

prey

V5 tag

Rbf 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rbf 
Rbf1 C-terminal fragment
sufficient binding region
aa 345-797
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0212298-1.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rbf 

Rbf

bait

GST tag

E2f1 

E2f1

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

FBrf0212298-3.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rbf 

Rbf

bait

FLAG tag

Rbf 
E2f1 

E2f1

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rbf 
pocket domain
necessary binding region
aa 376-727
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0223185-1.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rbf 

RBF1

bait

HA tag

E2f1 

E2F1

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

The white spot syndome virus proteins IE1 and WSV056 can bind to Rbf, displacing E2f. Consequently, E2f is activated and the G[[1]]/S transition is stimulated.

FBrf0227292-501.TFIN
Description
physical association
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
E2f1 
unspecified role
E2f1 
unspecified role
E2f1 
unspecified role
Rbf 
unspecified role
Rbf 
unspecified role
Rbf 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; proteins produced from transfected construct or endogenous gene.

HGScore = 30.835206

FBrf0238197-1.PD.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rbf 

RBF1

bait

GST tag

Rbf 
Rbf 
Rbf 
E2f1 

dE2F1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rbf 
LP domain
sufficient binding region
aa 376-845
Rbf 
SP domain
sufficient binding region
aa 376-711
Rbf 
C-terminal domain
sufficient binding region
aa 712-845
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The entire large-pocket, LP, domain of Rbf is required to efficiently bind the b isoform of E2f1 (E2f1-PD). The a isoform of E2f1 (E2f1-PA) binds efficiently to to both the small-pocket, SP, and C-terminal fragments of Rbf.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cell extract.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (8)