FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000095433
Interaction Network
Interactions Browser links
B52 network
tei network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0159267-11.SELEX
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
B52 

B52

bait
tei 

fas

prey
tei 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tei 
Intron 9
unspecified role
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed and purified in baculovirus-Sf9 cell system.

Prey derived from in vitro transcription of random primed genomic DNA PCR products.

B52-bound RNA was isolated by six rounds of SELEX and cDNAs were cloned.B52-bound RNA was isolated by six rounds of SELEX and cDNAs were cloned.

SELEX clones that mapped to the intron-containing genes were reported and further characterized.

FBrf0159267-32.EMSA.A
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
B52 

B52

bait
tei 

fas

prey

[32]P label

tei 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tei 
Intron 9
unspecified role
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed and purified in baculovirus-Sf9 cell system.

Prey derived from in vitro transcription.

B52-bound RNA was isolated by six rounds of SELEX and cDNAs were cloned.

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References (1)