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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000095541
Interaction Network
Interactions Browser links
h network
gro network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0076043-1.Y2H
Description
physical association
Assay
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
h 

hairy

bait

fused to LexA DNA-binding domain

gro 

Gro

prey

fused to B42 activation domain

gro 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gro 
C-terminal region
sufficient binding region
aa 251-719
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/B42-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

FBrf0076043-12.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
h 

hairy

bait

fused to LexA DNA-binding domain

h 
gro 

Gro

prey

fused to B42 activation domain

gro 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
h 
WRPW motif
necessary binding region
aa 334-337
gro 
central region
sufficient binding region
aa 251-414
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/B42-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

FBrf0076043-8.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
h 

Hairy

bait

GST tag

gro 

Gro

prey

[35]S label

gro 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gro 
C-terminal region
necessary binding region
aa 247-337
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

FBrf0088111-1.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
h 

Hairy

bait

GST tag

h 
h 
gro 

Groucho

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
h 
WRPW motif
sufficient binding region
WRPW
h 
WRPW motif
necessary binding region
WRPW
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

FBrf0088111-3.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
h 

Hairy

bait

fused to GAL4 DNA-binding domain

h 
h 
gro 

Groucho

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
h 
WRPW motif
sufficient binding region
WRPW
h 
WRPW motif
necessary binding region
WRPW
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/VP16-AD

FBrf0099085-1.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
h 

Hairy

bait

fused to LexA DNA-binding domain

gro 

Gro

prey

fused to VP16 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

h interacts with gro, but not with run or proneural genes sc, ac, ase or da.

Two-hybrid system: yeast GAL4-BD/VP16-AD

Positive control.

FBrf0099298-1.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
h 

Hairy

bait

GST tag

h 
gro 

Gro

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
h 
WRPW tetrapeptide
necessary binding region
WRPW
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

FBrf0102176-19.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gro 

Gro

bait

GST tag

h 

Hairy

prey

[35]S label

h 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
h 
WRPW motif
necessary binding region
aa 333-337

\'WRPW\' motif

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

FBrf0102176-9.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
h 

hairy

bait

fused to LexA DNA-binding domain

h 
h 
gro 

Gro

prey

fused to VP16 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
h 
C-terminal region
sufficient binding region
aa 299-343
h 
WRPW motif
necessary binding region
aa 334-337
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast LexA-BD/VP16-AD

FBrf0107533-1.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gro 

Groucho

bait

GST tag

h 

h

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

FBrf0109791-5.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
h 

Hairy

bait

GST tag

h 
gro 

Gro

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
h 
C-terminal region
necessary binding region
aa 247-337

"ΔNotI" construct

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

Positive control.

FBrf0125241-2.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
h 

Hairy

bait

GST tag

gro 

Gro

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed and purified from bacteria.

Prey derived from in vitro translation.

gro interacts more strongly with h than with cic.

GST pull down.

Positive control.

FBrf0139645-1.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
h 

Hairy

bait

GST tag

gro 

Groucho

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

Positive control.

FBrf0151327-2.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
h 

hairy

bait

GST tag

gro 

Gro

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

gro binds H (Hairless), h (hairy) but not Su(H).

Positive control.

FBrf0191885-2.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
h 

WRPW

bait

GST tag

h 
gro 

Gro

prey

[35]S label

gro 
gro 
gro 
gro 
gro 
gro 
gro 
gro 
gro 
gro 
gro 
gro 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
h 
WRPW motif
sufficient binding region
WRPW
gro 
WD domain residue 451
mutation disrupting interaction
aa 451

G451D, "MB35", coordinates relative to gro-PA

gro 
WD domain residue 483
mutation disrupting interaction
aa 483

R483H, "MB41", coordinates relative to gro-PA

gro 
WD domain residue 547
mutation disrupting interaction
aa 547

I547F, "MBD", coordinates relative to gro-PA

gro 
WD domain residue 570
mutation disrupting interaction
aa 570

C570Y, "MB19", coordinates relative to gro-PA

gro 
WD domain residue 646
mutation disrupting interaction
aa 646

H646P, "MB30", coordinates relative to gro-PA

gro 
WD domain residue 663
mutation disrupting interaction
aa 663

V663D, "E48", coordinates relative to gro-PA

gro 
WD domain residue 692
mutation disrupting interaction
aa 692

L692F, "MB31", coordinates relative to gro-PA

gro 
WD domain residue 651
mutation disrupting interaction
aa 651

L651F, coordinates relative to gro-PA

gro 
WD domain residue 481
mutation disrupting interaction
aa 481

Y481A, coordinates relative to gro-PA

gro 
WD domain residue 499
mutation disrupting interaction
aa 499

E499A, coordinates relative to gro-PA

gro 
WD domain residue 437
mutation disrupting interaction
aa 437

C437M, coordinates relative to gro-PA

gro 
WD domain residue 585
mutation disrupting interaction
aa 585

L585F, coordinates relative to gro-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

Mutations in gro that disrupt binding to the WRPW motif typically also disrupt binding of the eh1 motif (FxIxxIL), except for the L692F and L651F mutations, which disrupt only WRPW binding. The V435A and V435L mutations have no effect on gro binding to either the WRPW motif or eh1 domain.

A large scale genetic screen for enhancers of hairy recovered 50 novel gro alleles, including 7 mutations of the gro WD domain. These mutations, along with additional residues identified in a crystal structure of the human homolog TLE, were assessed for their effects on gro interactions with WRPW and eh1 repressor proteins.

FBrf0200831-4.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
h 

Hairy

bait

GST tag

gro 

Gro

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

FBrf0200831-6.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
h 

Hairy

bait

GST tag

gro 

Gro

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

FBrf0200898-5.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
h 

Hairy

bait

GST tag

gro 

Gro

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

Positive control.

FBrf0208965-3.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
h 

Hairy

bait

GST tag

gro 

Gro

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

FBrf0213366-11.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gro 

Gro

bait

GST

h 

Hairy

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Ubiquitylation of h protein abolishes interaction. Poly-monoubiquitylation of h protein by dgrn protein is sufficient to inhibit the interaction.

Cloned reagents; interaction in solution.

FBrf0213366-7.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
h 

Hairy

bait
h 
gro 

Gro

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
h 
WRPW domain
unspecified role

necessary

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Prey was cloned reagent (previously isolated).

Two-hybrid system: yeast LexA/VP16.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (15)