Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Co-expression of hh or smoCT diminished interaction
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Addition of PKAc reduced interaction suggesting PKAc binds Cos2 competitively with CkIα
Source was extract of S2 cells; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected construct.
Source was larval wing discs of transgenic fly line; bait produced from tagged transgenic construct; prey produced from transgenic construct.
Bait protein was fused to the motor domain of kinesin heavy chain (Khc), which targets the chimera to the plus end of microtubules. The ability of this chimera to recruit prey protein to the plus end of microtubules was taken as evidence of direct physical interaction.
This interaction is inhibited by the co-expression of activated fu constructs.
Bait expressed in transiently transfected cells.
Prey expressed in transiently transfected cells.
