A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Tor, Lst8 Interaction report

General Information
Interaction Type
physical association
Interacting Genes
Tor, Lst8
FlyBase ID
FBig0000095655
Interaction Network
Interactions Browser links
Tor network

Lst8 network
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hideFBrf0218417-1.coIP.WB
Reference
(Wang et al., 2012)
Description
physical association
Assay
anti bait coimmunoprecipitation, anti tag western blot
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tor protein
TOR
bait
Myc tag
Lst8 protein
LST8
prey
FLAG tag
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
 
The interaction of Lst8 with Tor does not require co-expression of raptor. Bait derived from transgenic line carrying a Myc-tagged Tor construct, P{UAS-Tor.myc}. Prey derived from transgenic line carrying a FLAG-tagged Lst8 construct, P{UAS-Lst8.flag}.
hideFBrf0218417-3.coIP.WB
Reference
(Wang et al., 2012)
Description
physical association
Assay
anti bait coimmunoprecipitation, anti tag western blot
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Lst8 protein
LST8
bait
FLAG tag
Tor protein
TOR
prey
Myc tag
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
 
The interaction of Lst8 with Tor does not require co-expression of raptor. Bait derived from transgenic line carrying a FLAG-tagged Lst8 construct, P{UAS-Lst8.flag}. Prey derived from transgenic line carrying a Myc-tagged Tor construct, P{UAS-Tor.myc}.
hide References ( 0 )
 
No references are recorded in FlyBase at present.