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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000095712
Interaction Network
Interactions Browser links
smo network
Usp8 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0217210-2.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
smo 

smo

bait

Myc tag

smo 
smo 
Usp8 

Usp8

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
smo 
C-terminal domain
sufficient binding region
aa 556-1035
smo 
C-terminal domain
necessary binding region
aa 556-1035
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0218247-1.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait

Myc tag

smo 
Usp8 

USP8

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
smo 
C-terminal region
sufficient binding region
aa 556-730aa 731-1035

weak interaction

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed in transfected cells.

Prey expressed in transfected cells.

FBrf0218247-2.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait

Myc tag

smo 
smo 
smo 
Usp8 

USP8

prey

HA tag

Usp8 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
smo 
C-terminal region
sufficient binding region
aa 556-1035
smo 
PKA phosphorylation sites
mutation decreasing interaction
aa 667-740

S667A, S687A and S740A mutations abolish interaction

smo 
PKA and CK1 phosphorylation sites
mutation increasing interaction
aa 667-743

mutation of Serine residues to Aspartate increases interaction

Usp8 
C-terminal region
sufficient binding region
aa 536-896
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed in transfected cells.

Prey expressed in transfected cells.

Mutations that mimic the phosphorylated state the smo C-terminal region enhance the smo-Ubpy interaction.

FBrf0218247-3.PD.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait

GST tag

smo 
smo 
Usp8 

USP8

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
smo 
C-terminal region
sufficient binding region
aa 625-753
smo 
PKA and CK1 phosphorylation sites
mutation increasing interaction
aa 667-743

mutation of Serine residues to Aspartate increases interaction

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

GST pull down.

Bait expressed and purified from bacteria.

Prey expressed in transfected cells.

Treatment of S2 cells with hh-containing conditioned medium increases the interaction of Ubpy with GST-smo.

Mutations that mimic the phosphorylated state the smo C-terminal region enhance the smo-Ubpy interaction.

FBrf0223342-3.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait

Myc tag

Usp8 

USP8

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

The binding of Ubpy to smo is not affected by knockdown or over-expression of Hrs.

FBrf0235873-2.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait

Myc tag

Usp8 

Usp8

prey

FLAG tag

Usp8 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Usp8 
interaction domain
mutation disrupting interaction
aa 260,262,263,498,500,501,780,782,783

I260K,I262K,V263K,V498K,V500K,V501K,V780K,V782K,V783K

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)