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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000095794
Interaction Network
Interactions Browser links
eIF4E1 network
Thor network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0184136-7.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

4E1

bait

fused to GAL4 DNA-binding domain

Thor 

4EBP

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

FBrf0216604-35.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Thor 

Thor

bait

GFP tag

eIF-4E

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

SAINT score = 1

The Thor-eIF-4E interaction decreases to 0.3-fold of untreated baseline upon insulin stimulation.

Source was cell extract of Kc167 cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0219080-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

eIF4E-1

bait
Thor 

4E-BP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait endogenous to testis extract.

Prey endogenous to testis extract.

Thor binds eIF-4E but not eIF4E-3 in testis.

Coimmunoprecipitation done in the presence of RNaseA.

FBrf0219080-2.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Thor 

4E-BP

bait

fused to GAL4 DNA-binding domain

eIF4E-1

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Thor interacts with eIF-4E, eIF4E-4 and eIF4E-5, but not with eIF4E-3, in the yeast two-hybrid system.

Two-hybrid system: yeast GAL4-BD/GAL4-AD.

FBrf0222078-1.FW.A
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note

eIF4E-1

bait

[32]P label

Thor 

4E-BP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

FBrf0222078-9.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

eIF4E-1

bait

HA tag

Thor 

4E-BP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

CG2950 (mxt) exhibited the strongest affinity for eIF-4E, compared to eIF4G and Thor.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0226072-10.PD.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Thor 

Thor

bait

MBP tag

Thor 

eIF4E

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
C-terminal region
sufficient binding region
aa 69-248

coordinates relative to eIF4E1-PC

Thor 
eIF4E-binding region
sufficient binding region
aa 50-83

coordinates relative to Thor-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

FBrf0226072-15.ITC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

eIF4E

bait

His tag

Thor 

Thor

prey

GB1 tag

Thor 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
C-terminal region
sufficient binding region
aa 69-248

coordinates relative to eIF4E1-PC

Thor 
eIF4E-binding region
sufficient binding region
aa 50-83

coordinates relative to Thor-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

Kd = 1.4 nM

FBrf0226072-3.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

eIF4E

bait

HA tag

Thor 

Thor

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dorsal binding surface residue W106
mutation disrupting interaction
aa 106

W106A, coordinates relative to eIF4E1-PC

lateral binding surface residues I96 and I112
mutation disrupting interaction
aa 96-112

"II-AA" construct (I96A + I112A), coordinates relative to eIF4E1-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Mutation of residues on the lateral surface of eIF4E1 (I96A and I112A) specifically disrupts interaction with 4E-BP-type proteins (Thor, cup and 4E-T) but does not affect binding to eIF4G1.

FBrf0226072-7.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Thor 

Thor

bait

HA tag

Thor 
Thor 
Thor 

eIF4E

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Thor 
canonical 4E-binding motif residues Y54 and M59
mutation disrupting interaction
aa 54-59

"C*" construct (Y54A + M59A), coordinates relative to Thor-PA

Thor 
non-canonical 4E-binding motif residues
necessary binding region
aa 76-84

"ΔNC" construct, coordinates relative to Thor-PA

Thor 
non-canonical 4E-binding motif residues C78-L80
mutation decreasing interaction
aa 78-80

"NC*" construct (C78A + L79A + L80A), coordinates relative to Thor-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0229498-5.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

eIF4E

bait

HA tag

Thor 

Thor

prey

GFP

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0233454-1627.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Thor 

Thor

bait

TAP tag

eIF-4E

prey

SAINT score = 1

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

Observed in all three conditions tested: uninduced cells, 10 minutes after insulin stimulation and 30 minutes after insulin stimulation.

FBrf0233905-5.PD.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

eIF4E

bait

His tag

Thor 

Thor

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
eIF4E binding region
sufficient binding region
aa 58-83

"C+L+NC" construct, coordinates relative to Thor-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey peptide synthesized in vitro.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (8)