FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Thor, eIF4E1
FlyBase ID
FBig0000095794
Interaction Network
Interactions Browser links
Thor network
eIF4E1 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0184136-7.Y2H
Reference
Hernandez et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
eIF4E1 

4E1

bait

fused to GAL4 DNA-binding domain

Thor 

4EBP

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Hernandez et al., 2005)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Hernandez et al., 2005)
FBrf0216604-35.coIP.MS
Reference
Glatter et al., 2011
Description
physical association
Assay
Collection
PPI_InR-Tor
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
Thor 

Thor

bait

GFP tag

eIF4E1 

eIF-4E

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

SAINT score = 1

(Glatter et al., 2011)

The Thor-eIF-4E interaction decreases to 0.3-fold of untreated baseline upon insulin stimulation.

(Glatter et al., 2011)

Source was cell extract of Kc167 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Glatter et al., 2011)
FBrf0219080-1.coIP.WB
Reference
Hernandez et al., 2012
Description
physical association
Assay
Collection
Source/Stage
testis / adult stagemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
eIF4E1 

eIF4E-1

bait
Thor 

4E-BP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait endogenous to testis extract.

(Hernandez et al., 2012)

Prey endogenous to testis extract.

(Hernandez et al., 2012)

Thor binds eIF-4E but not eIF4E-3 in testis.

(Hernandez et al., 2012)

Coimmunoprecipitation done in the presence of RNaseA.

(Hernandez et al., 2012)
FBrf0219080-2.Y2H
Reference
Hernandez et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Thor 

4E-BP

bait

fused to GAL4 DNA-binding domain

eIF4E1 

eIF4E-1

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Thor interacts with eIF-4E, eIF4E-4 and eIF4E-5, but not with eIF4E-3, in the yeast two-hybrid system.

(Hernandez et al., 2012)

Two-hybrid system: yeast GAL4-BD/GAL4-AD.

(Hernandez et al., 2012)
FBrf0222078-1.FW.A
Reference
Hernández et al., 2013
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 17
Cell line used
Participants
Corresponds to
Reported as
Role
Note
eIF4E1 

eIF4E-1

bait

[32]P label

Thor 

4E-BP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

(Hernández et al., 2013)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Hernández et al., 2013)
FBrf0222078-9.coIP.WB
Reference
Hernández et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
eIF4E1 

eIF4E-1

bait

HA tag

Thor 

4E-BP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

(Hernández et al., 2013)

CG2950 (mxt) exhibited the strongest affinity for eIF-4E, compared to eIF4G and Thor.

(Hernández et al., 2013)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Hernández et al., 2013)
FBrf0226072-10.PD.MW
Reference
Igreja et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Thor 

Thor

bait

MBP tag

Thor 
eIF4E1 

eIF4E

prey

His tag

eIF4E1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
eIF4E1 
C-terminal region
sufficient binding region
aa 69-248

coordinates relative to eIF4E1-PC

Thor 
eIF4E-binding region
sufficient binding region
aa 50-83

coordinates relative to Thor-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Igreja et al., 2014)
FBrf0226072-15.ITC
Reference
Igreja et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
eIF4E1 

eIF4E

bait

His tag

eIF4E1 
Thor 

Thor

prey

GB1 tag

Thor 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
eIF4E1 
C-terminal region
sufficient binding region
aa 69-248

coordinates relative to eIF4E1-PC

Thor 
eIF4E-binding region
sufficient binding region
aa 50-83

coordinates relative to Thor-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Igreja et al., 2014)

Kd = 1.4 nM

(Igreja et al., 2014)
FBrf0226072-3.coIP.WB
Reference
Igreja et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
eIF4E1 

eIF4E

bait

HA tag

eIF4E1 
eIF4E1 
Thor 

Thor

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
eIF4E1 
dorsal binding surface residue W106
mutation disrupting interaction
aa 106

W106A, coordinates relative to eIF4E1-PC

eIF4E1 
lateral binding surface residues I96 and I112
mutation disrupting interaction
aa 96-112

"II-AA" construct (I96A + I112A), coordinates relative to eIF4E1-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Igreja et al., 2014)

Mutation of residues on the lateral surface of eIF4E1 (I96A and I112A) specifically disrupts interaction with 4E-BP-type proteins (Thor, cup and 4E-T) but does not affect binding to eIF4G1.

(Igreja et al., 2014)
FBrf0226072-7.coIP.WB
Reference
Igreja et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Thor 

Thor

bait

HA tag

Thor 
Thor 
Thor 
eIF4E1 

eIF4E

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Thor 
canonical 4E-binding motif residues Y54 and M59
mutation disrupting interaction
aa 54-59

"C*" construct (Y54A + M59A), coordinates relative to Thor-PA

Thor 
non-canonical 4E-binding motif residues
necessary binding region
aa 76-84

"ΔNC" construct, coordinates relative to Thor-PA

Thor 
non-canonical 4E-binding motif residues C78-L80
mutation decreasing interaction
aa 78-80

"NC*" construct (C78A + L79A + L80A), coordinates relative to Thor-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Igreja et al., 2014)
FBrf0229498-5.coIP.WB
Reference
Peter et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
eIF4E1 

eIF4E

bait

HA tag

Thor 

Thor

prey

GFP

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Peter et al., 2015)
FBrf0233454-1627.coIP.MS
Reference
Vinayagam et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Thor 

Thor

bait

TAP tag

eIF4E1 

eIF-4E

prey

SAINT score = 1

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Vinayagam et al., 2016)

Observed in all three conditions tested: uninduced cells, 10 minutes after insulin stimulation and 30 minutes after insulin stimulation.

(Vinayagam et al., 2016)
FBrf0233905-5.PD.MW
Reference
Grüner et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
eIF4E1 

eIF4E

bait

His tag

eIF4E1 
Thor 

Thor

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
eIF4E1 
eIF4E binding region
sufficient binding region
aa 58-83

"C+L+NC" construct, coordinates relative to Thor-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey peptide synthesized in vitro.

(Grüner et al., 2016)
FBrf0250268-1.XC
Reference
Peter et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
eIF4E1 

eIF4E

neutral component
eIF4E1 

eIF4E

neutral component
eIF4E1 
eIF4E1 

eIF4E

neutral component
eIF4E1 
Thor 

Thor

neutral component
Thor 
Thor 

Thor

neutral component
Thor 

Thor

neutral component
Thor 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
eIF4E1 
interaction domain
sufficient binding region
aa 1-248

coordinates relative to eIF4E1-PC

Thor 
interaction domain
sufficient binding region
aa 1-117

coordinates relative to Thor-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Peter et al., 2015)
FBrf0250268-10.ITC
Reference
Peter et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
eIF4E1 

eIF4E

bait
eIF4E1 
Thor 

Thor

prey
Thor 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Thor 
interaction domain
sufficient binding region
aa 50-83

coordinates relative to Thor-PA.

eIF4E1 
interaction domain
sufficient binding region
aa 69-248
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Peter et al., 2015)
FBrf0250268-4.PD.MW
Reference
Peter et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Thor 

Thor

bait

MBP tag

Thor 
Thor 
Thor 
eIF4E1 

eIF4E

prey

Strep-His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Thor 
canonical and non-canonical domains
mutation disrupting interaction
aa 54, 59, 75 and 79

Y54A, M59A, V75D, L79D; within the context of aa 50-83; coordinates relative to Thor-PA

Thor 
structurally conserved residues
mutation disrupting interaction
aa 56, 63, 81

R56E, R63E, R81E, within the context of aa 50-83; coordinates relative to Thor-PA

Thor 
linker and canonical domains
mutation disrupting interaction
aa 65, 70, 54 and 59

Y54A, M59A, S65D, T70D, within the context of aa 32-83; coordinates relative to Thor-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait and prey produced as recombinant fusion proteins in bacterial system.

(Peter et al., 2015)

Mutation of Thor linker residues, within the context of the full-length protein, disrupts interaction of Thor-eIF4E1 only in the presence of eIF4G1.

(Peter et al., 2015)
FBrf0250268-7.coIP.WB
Reference
Peter et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Thor 

Thor

bait

HA tag

Thor 
Thor 
eIF4E1 

eIF4E1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Thor 
canonical domain residues 54 and 59
necessary binding region
aa 54 and 59

Y54A, M59A, within the context of aa 50-83; coordinates relative to Thor-PA

Thor 
non-canonical domain residues 75 and 79
necessary binding region
aa 75 and 79

V75D, L79D; within the context of aa 50-83; coordinates relative to Thor-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Peter et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (9)