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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000096836
Interaction Network
Interactions Browser links
MYPT-75D network
flw network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0180501-3.Y2H
Description
physical association
Assay
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
flw 

Pp1β9C

bait

fused to GAL4 DNA-binding domain

MYPT-75D

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

MYPT-75D discriminates between PP1 protein phosphotases, binding to flw but not to Pp1-87B.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from larval cDNA expression library).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

FBrf0180501-5.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

MYPT75D

bait

Myc tag

flw 

PP1β9C

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
RVXF motif
mutation disrupting interaction
aa 117

F117A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adults of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

MYPT-75D coimmunoprecipitates flw (Pp1β9C) but not Pp1-87B.

FBrf0180501-8.ENZ
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
flw 

PP1β9C

enzyme

His tag

flw 

PP1β9C

enzyme

His tag

MYPT75D

stimulator

His tag

MYPT75D

stimulator

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0194330-33.Y2H
Description
physical association
Assay
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
flw 

PP1β9C

bait

fused to GAL4 DNA-binding domain

flw 

MYPT-75D

prey

fused to VP16 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
flw 
C-terminal domain
necessary binding region
aa 304-330
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from third instar larva cDNA expression library).

Binding of MYPT-75D to the C-terminal domain of the protein phosphatase flw is not typical of other most other interactions between protein phosphatases and their regulatory subunits.

Two-hybrid system: yeast GAL4-BD/VP16-AD

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)