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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000096968
Interaction Network
Interactions Browser links
Prosα4 network
PI31 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0221396-7.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

α4

bait

FLAG tag

PI31 

DmPI31

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0221396-9.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

α4

bait

FLAG tag

PI31 

DmPI31

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

ADP-ribosylation of PI31 reduces its interaction with Prosα4 and decreases PI31 inhibition of the 20S proteasome in vitro.

Interaction in vitro; bait produced as a recombinant fusion protein in HEK293 cells; bait produced as recombinant fusion protein in bacterial system.

PI31 was ADP-ribosylated in vitro by tankyrase.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)