FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
grk, BicC
FlyBase ID
FBig0000097051
Interaction Network
Interactions Browser links
grk network
BicC network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0218722-6.PD.MS
Reference
McDermott et al., 2012
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stagefemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
grk 

GLS

bait

λ phage BoxB tag linked to GST

grk 
BicC 

BicC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
grk 
gurken localization signal
necessary binding region
nt 406-470
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced by in vitro transcription; prey derived from wild-type ovary extract.

(McDermott et al., 2012)

GRNA chromatography: grk RNA containing a BoxB sequence of the λ phage was bound to a fusion of GST with the 21 residue N antiterminator peptide.

(McDermott et al., 2012)

The grk-GST particles were pre-bound to resin and incubated with ovary extract.

(McDermott et al., 2012)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)