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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000097171
Interaction Network
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0104394-2.Y2H
Description
physical association
Assay
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ena 

EnaC

bait

fused to GAL4 DNA-binding domain

ena 

EnaC

prey

fused to GAL4 activation domain

ena 
ena 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ena 
C-terminal region
sufficient binding region
aa 441-684
ena 
EVH2 domain
sufficient binding region
aa 628-684
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was from larval cDNA expression library).

A portion of ena containing residues 441-684 interacts with another portion of ena containing residues 628-684.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

FBrf0104394-3.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ena 

Ena

bait

His tag

ena 

Ena

prey

FLAG tag

ena 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ena 
EVH2 domain
necessary binding region
aa 636-684
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0108135-2.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ena 

Ena

bait

fused to GAL4 DNA-binding domain

ena 

Ena

prey

fused to GAL4 activation domain

ena 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ena 
C-terminal domain
sufficient binding region
aa 374-684

coordinates relative to ena-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from third instar larval cDNA expression library).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

External Crossreferences and Linkouts ( 0 )
References (2)