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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000097225
Interaction Network
Interactions Browser links
yki network
sd network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0204309-1.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sd 

SD

bait

GST tag

sd 
yki 

YKI

prey

[35]S label

yki 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sd 
C-terminal region
sufficient binding region
aa 212-440

coordinates relative to sd-PB

yki 
N-terminal region
sufficient binding region
aa 1-127

coordinates relative to yki-PH

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

FBrf0204632-1.Y2H
Reference
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

fused to hSos GEF protein

yki 
sd 

Sd

prey

fused to myristoylation signal

sd 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
yki 
N-terminal homology domain
sufficient binding region
aa 47-151

coordinates relative to yki-PH

sd 
C-terminal region
sufficient binding region
aa 226-440

coordinates relative to sd-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

7 sd clones were recovered in the yki screen.

Two-hybrid system: yeast Stratagene CytoTrap

Interaction of prey with bait targets hSos domain to membrane, activating Ras and permitting mutant cdc25H yeast to grow at 37oC.

FBrf0204632-2.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

HA tag

yki 
sd 

Sd

prey

HSV tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
yki 
N-terminal homology domain residue P88
mutation disrupting interaction
aa 88

P88L, coordinates relative to yki-PH

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0204676-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sd 

Sd

bait

HA tag

sd 
yki 

Yki

prey

MYC tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sd 
C terminal region
sufficient binding region
aa 227-440

coordinates relative to sd-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0204676-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

MYC tag

sd 

Sd

prey

HA tag

sd 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sd 
C terminal region
sufficient binding region
aa 227-440

coordinates relative to sd-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0221209-1.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
yki 

YKI

bait

GST tag

sd 

SD

prey

[35]S label

sd 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sd 
region
mutation disrupting interaction
aa 303

W303A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

FBrf0221209-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sd 

SD

bait

FLAG tag

yki 

YKI

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of 293T cell line; bait produced from transfected construct; prey produced from transfected construct.

vg outcompetes yki for binding to sd when the three proteins are coexpressed.

FBrf0222482-10.coIP.MS
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait
sd 

Sd

prey

1 unique peptide identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The majority of yki protein is cytoplasmic. To avoid isolating these cytoplasmic factors and instead identify proteins involved in yki transcriptional functions, yki was immunoprecipitated from nuclear extracts.

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0224345-156.coIP.MS
Description
physical association
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sd 

sd

bait

Protein A tag, Strep tag

yki 

yki

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0224345-201.coIP.MS
Description
physical association
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
yki 

yki

bait

Protein A tag, Strep tag

sd 

sd

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0224710-14.PD.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sd 

Sd

bait

GST tag

yki 

Yki

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

The sd-yki interaction is not affected by the presence of wild-type Tgi. However, a Tgi variant carrying three disabled PPXY motifs does disrupt the sd-yki interaction.

FBrf0224710-15.FRET
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sd 

Sd

bait

CFP tag

yki 

Yki

prey

YFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

FRET efficiency between sd and yki was decreased upon cotransfection of Tgi.

Source was live S2 cells; bait produced from transfected construct; prey produced from transfected construct.

FBrf0224710-2.Y2H
Reference
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sd 

Sd

bait

fused to GAL4 DNA-binding domain

sd 
yki 

Yki

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sd 
C-terminal region
sufficient binding region
aa 208-440
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

Two-hybrid system: yeast LexA-BD/GAL4-AD

Positive control.

FBrf0224710-8.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

Myc tag

sd 

Sd

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Sequential coimmunoprecipitation, first of FLAG-Tgi, then of Myc-yki, in the presence of HA-sd, suggests that sd, yki and Tgi form a ternary complex.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0224710-9.Y2H
Reference
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

fused to GAL4 DNA-binding domain

sd 

Sd

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

Two-hybrid system: yeast LexA-BD/GAL4-AD

Positive control.

FBrf0225127-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sd 

sd

bait

HA tag

yki 

yki

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Mutant form of yki S168A used to show

FBrf0229775-3.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sd 

Sd

bait

HSV tag

yki 

Yki

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Increased expression of Tgi results in a decrease in binding between sd and yki in a dose-dependent manner.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0229775-4.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sd 

Sd

bait

HSV tag

yki 

Yki

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Increased expression of vg results in a decrease in binding between sd and yki in a dose-dependent manner.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0237426-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sd 

Sd

bait

HA tag

yki 

Yki

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

The sd-yki interaction decreases upon coexpression of E2f1.

FBrf0237426-4.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sd 

Sd

bait

HA tag

yki 

Yki

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

The sd-yki interaction decreases upon coexpression of E2f1.

FBrf0240957-2.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

Myc tag

sd 

Sd

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Coexpression of CG7028 reduces the yki-sd interaction.

FBrf0241879-3.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sd 

Sd

bait

FLAG tag

yki 

Yki

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0246490-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
yki 

yki

bait

GFP tag

sd 

Sd

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

Co-expression of the vg TONDU domain peptide inhibits the yki-sd interaction.

FBrf0246989-2.SPR
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sd 

Sd

bait
sd 
sd 
sd 
yki 

Yki

prey
yki 
yki 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sd 
interaction domain
sufficient binding region
aa 223-440

coordinates relative to sd-PB

yki 
β-strand:α-helix and Ω-loop domains
sufficient binding region
aa 31-79

Kd = 23nM, coordinates relative to yki-PD

yki 
α-helix and Ω-loop domains
sufficient binding region
aa 41-79

Kd = 69nM, coordinates relative to yki-PD

sd 
interaction domain residue 276
mutation decreasing interaction
aa 276

D276A, coordinates relative to sd-PB

sd 
interaction domain residue 435
mutation decreasing interaction
aa 435

Y435H, coordinates relative to sd-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

FBrf0247927-4.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

Firefly Luciferase tag

sd 

Sd

prey

Renilla Luciferase tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

No difference in yki-sd interaction with different yki isoforms including or excluding exon 3.

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (15)