Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

The SNAP/PBP complex was isolated from soluble nuclear extract by fractionation on DEAE-cellulose and heparin columns.

DNA-binding subunits within the SNAP/PBP complex were detected by photo-cross-linking and molecular weight estimation of proteins on a gel. The identity of these proteins was later determined by using antibodies and supershift assays in a subsequent study (FBrf0174956).

Pbp45, Pbp49 and Pbp95 are part of the SNAP (small nuclear RNA activating protein) complex, also known as the PBP (PSE binding protein) complex, involved in snRNA transcription.

Interaction in vitro; bait produced as recombinant fusion protein in transfected S2 cell extract; prey produced from endogenous gene.

Pbp45-containing complexes were affinity purified from transfected S2 cell extracts and used in electromobility shift assays using [32]P labeled DNA containing the U1 snRNA \'PSEA\' motif. The presence of other proteins in the Pbp45-DNA complex was assessed using antibodies to supershift the apparent migration of the protein:DNA complex.

Pbp45, Pbp49 and Pbp95 are part of the SNAP (small nuclear RNA activating protein) complex, also known as the PBP (PSE binding protein) complex, involved in snRNA transcription.

Interaction in vitro; bait produced as recombinant fusion protein in transfected S2 cell extract; prey produced from endogenous gene.

Pbp49-containing complexes were affinity purified from transfected S2 cell extracts and used in electromobility shift assays using [32]P labeled DNA containing the U1 snRNA \'PSEA\' motif. The presence of other proteins in the Pbp49-DNA complex was assessed using antibodies to supershift the apparent migration of the protein:DNA complex.

The SNAP complex (Pbp45, Pbp49 AND Pbp95) has apparent molecular mass of 372 kDa.

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Pbp95 is coexpressed with Pbp45 and Pbp49.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Pbp95 is coexpressed with Pbp45 and Pbp49.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Pbp45, Pbp49, Pbp95 form trimeric complex. Captured as pairwise interactions.