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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000097900
Interaction Network
Interactions Browser links
mle network
msl-1 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0104740-7.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mle 

MLE1

bait

MSL1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of SL2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

Weak coimmunoprecipitation of MSL proteins compared to the amount of mle immunoprecipitated, suggesting that only a fraction of mle is associated with MSL proteins.

FBrf0123205-10.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

MSL1

bait
mle 

MLE

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

The interaction of mle with msl-1 is reduced by brief treatment with 0.4 M NaCl.

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0202987-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mle 

MLE

bait

FLAG tag

MSL1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0222154-4.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

MSL1

bait
mle 

MLE

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The interaction of mle with msl-1 is much weaker than that of MSL complex components (msl-2, msl-3 and mof). In contrast to interactions between MSL complex components, only a small fraction of mle coimmunoprecipitates with msl-1 and the interaction is disrupted by RNaseA treatment. Nonetheless, depletion of mle leads to reduction in MSL proteins and roX1 and roX2 RNA levels.

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0222216-6.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Msl1

bait

FLAG tag

mle 

Mle

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

Source was cell extract of SL2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0225790-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mle 

MLE

bait

FLAG tag

MSL1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

The interaction of msl-1 with mle is RNA-dependent (disrupted by RNase treatment).

The interaction of msl-1 with mle is not disrupted by roX2-directed RNAi.

Positive control.

FBrf0229405-16.PLA
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note

msl-1

neutral component

msl-1

neutral component

msl-1

neutral component
mle 

mle

neutral component
mle 

mle

neutral component
mle 

mle

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Factors were detected on polytene chromosomes using antibodies (to proteins) or biotinylated antisense probes (to ncRNA). The PLA signal develops only if probes to the two factors are in close proximity to each other (within tens of nanometers).

Source was larval salivary gland of wild-type fly line; proteins produced from endogenous gene.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (7)