Interaction in vitro; enzyme produced from transfected construct in HEK293T cell line.
hipk is autophosphorylated.
Enzyme was expressed in and coimmunoprecipitated from HEK293T extract for the in vitro kinase assay. Transfer of [32]P from ATP to proteins monitored by autoradiography.
Interaction in vitro; enzyme produced as a recombinant fusion protein in bacterial system.
In vitro kinase assay. Autophosphorylation.
