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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000098131
Interaction Network
Interactions Browser links
Atx2 network
pAbp network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0193060-1.CS.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Atx2 

ATX2

neutral component
Atx2 

ATX2

neutral component
Atx2 

ATX2

neutral component
pAbp 

dPABP

neutral component
pAbp 

dPABP

neutral component
pAbp 

dPABP

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of wild-type fly line; proteins produced from endogenous genes.

FBrf0193060-2.CS.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Atx2 

ATX2

neutral component
Atx2 

ATX2

neutral component
Atx2 
Atx2 
Atx2 
Atx2 

ATX2

neutral component
Atx2 
Atx2 
Atx2 
pAbp 

dPABP

neutral component
pAbp 

dPABP

neutral component
pAbp 

dPABP

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Atx2 
N-terminal domain
sufficient binding region
aa 1-298

coordinates relative to Atx2-PB

Atx2 
C-terminal domain
sufficient binding region
aa 299-1084

coordinates relative to Atx2-PB

Atx2 
PAM2 domain
necessary binding region
aa 855-869

coordinates relative to Atx2-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; Atx2 produced from transfected construct: pAbp produced from endogenous gene.

FBrf0193060-3.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Atx2 

ATX2

bait
pAbp 

dPABP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0193060-4.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Atx2 

ATX2

bait

FLAG tag

Atx2 
Atx2 
pAbp 

dPABP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Atx2 
C-terminal domain
sufficient binding region
aa 299-1084

coordinates relative to Atx2-PB

Atx2 
PAM2 domain
necessary binding region
aa 855-869

coordinates relative to Atx2-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0221600-7.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Atx2 

ATX2

bait

FLAG tag

Atx2 
Atx2 
pAbp 

PABP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Atx2 
C-terminal region
sufficient binding region
aa 785-1084

\'ATX2-C\'

Atx2 
PABP-interacting motif 2
necessary binding region
aa 855-869

\'ATX2ΔPAM2\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

tyf and pAbp separately bound to different regions of Atx2 in a ribonuclease-insensitive manner.

FBrf0221600-9.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Atx2 

ATX2

bait

FLAG tag

Atx2 
pAbp 

PABP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Atx2 
PABP-interacting motif 2
necessary binding region
aa 855-869

\'ATX2ΔPAM2\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult head of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

FBrf0235227-19.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Atx2 

Atx2

bait
pAbp 

pAbp

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0235227-2.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Atx2 

Atx2

bait
pAbp 

pAbp

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

Interaction not disrupted by RNase treatment.

FBrf0235227-27.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Atx2 

Atx2

bait

FLAG tag

Atx2 
pAbp 

pAbp

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Atx2 
interaction domain
sufficient binding region
aa 1-298
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)