Binding requires divalent cations (Ca[++] or Mg[++]).

Interaction in vitro; bait produced as a recombinant fusion protein in COS cell system; prey produced as a recombinant fusion protein in COS cell system.

Transmembrane bait protein was obtained from crude membrane preparations of transfected cells. Prey protein was obtained from cell culture supernatants of transfected cells. Proteins were mixed in vitro.

Interaction in vitro; bait derived from conditioned medium of transfected S2 cell line; prey derived from conditioned medium of transfected S2 cell line.

Positive control.

Interaction in vitro; bait derived from conditioned medium of transfected S2 cell line; prey derived from conditioned medium of transfected S2 cell line.

Positive control.

Kd = 586nM.

Interaction in vitro; bait produced as a recombinant fusion protein in HEK293 cells; prey produced as a recombinant fusion protein in HEK293 cells.

PlexA-Sema1b forms two different complexes in crystallo - a 1:1 complex and a 2:2 complex with distinct interaction modes.

Interaction in vitro; proteins purified as recombinant fusion proteins from conditioned medium of transfected HEK 293T cells.