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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000098224
Interaction Network
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0221091-13.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Duf

bait

fused to LexA DNA-binding domain

dock 

Dock

prey

fused to B42 activation domain

dock 
dock 
dock 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
intracellular domain
sufficient binding region
dock 
SH3 domain 1
necessary binding region
dock 
SH3 domain 2
necessary binding region
dock 
SH3 domain 3
necessary binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/B42-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

FBrf0221091-14.FC.FM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dock 

Dock

neutral component

N-terminal YFP tag

dock 

Dock

neutral component

N-terminal YFP tag

dock 
dock 

Dock

neutral component

N-terminal YFP tag

dock 

Duf

neutral component

C-terminal YFP tag

Duf

neutral component

C-terminal YFP tag

Duf

neutral component

C-terminal YFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
intracellular domain
sufficient binding region
proline-rich region
necessary binding region
aa 810-956
dock 
SH3 domains
necessary binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cells S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0223688-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Duf

bait

GFP tag

dock 

Dock

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Src phosphorylation site
mutation disrupting interaction
aa 810

Y810A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)