FB2020_06, released Dec 22, 2020

Physical Interaction report

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General Information
Interaction Type
physical association
Interacting Genes
sli, robo2
FlyBase ID
FBig0000098228
Interaction Network
Interactions Browser links
sli network
robo2 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0132431-1.SPA.TVEA
Reference
Simpson et al., 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sli 

Slit

bait
robo2 

Robo2

prey

AP tag

robo2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
robo2 
ectodomain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait produced from transfected construct in COS cell line; prey produced from cell extract of transfected construct in a 293 cell line.

(Simpson et al., 2000)
FBrf0180200-6.ELISA
Reference
Howitt et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sli 

Slit

bait

His, Myc tag

sli 
sli 
sli 
sli 
sli 
robo2 

Robo2

prey

Fc tag

robo2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
robo2 
IG domains 1-5
sufficient binding region
aa 24-528

"D1-5", coordinates relative to robo2-PA

sli 
LRR domain 2
sufficient binding region
aa 314-542

"D2", coordinates relative to sli-PC

sli 
LRR domain 2 residue Y402
mutation disrupting interaction
aa 402

Y402S, coordinates relative to sli-PC

sli 
LRR domain 2 residue L424
mutation disrupting interaction
aa 424

L424R, coordinates relative to sli-PC

sli 
LRR domain 2 residue Y450
mutation disrupting interaction
aa 450

Y450S, coordinates relative to sli-PC

sli 
LRR domain 2 residue H472
mutation disrupting interaction
aa 472

H472S, coordinates relative to sli-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait purified from conditioned medium of transfected 293 cells; prey purified from conditioned medium of transfected 293 cells.

(Howitt et al., 2004)
FBrf0210412-2.SPR
Reference
Evans and Bashaw, 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
robo2 

Robo2

neutral component

His tag

robo2 

Robo2

neutral component

His tag

robo2 
robo2 

Robo2

neutral component

His tag

robo2 
sli 

Slit

neutral component

His tag

sli 
sli 

Slit

neutral component

His tag

sli 

Slit

neutral component

His tag

sli 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
robo2 
Ig domains 1-5
sufficient binding region
sli 
Robo-binding domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus system; prey produced as a recombinant fusion protein in baculovirus system.

(Evans and Bashaw, 2010)

sli binds robo (Kd = 235nM) more strongly than lea/robo2 (Kd = 1098nM).

(Evans and Bashaw, 2010)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)