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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000098282
Interaction Network
Interactions Browser links
msl-3 network
mof network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0129699-4.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mof 
MOF
bait
MSL3
prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
This interaction persists upon RNase treatment.
The interaction of msl-3 with mof is not disrupted by high salt.
Source was cell extract of SL2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.
FBrf0179765-3.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
MSL3
bait
FLAG tag
mof 
MOF
prey
HA tag
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
The msl-3-mof interaction is detected only in the presence of msl-1.
Source was cell extract of baculovirus-infected Sf9 cell line; bait produced from transfected construct; prey produced from transfected construct.
FBrf0179765-5.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mof 
MOF
bait
HA tag
MSL3
prey
FLAG tag
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
The msl-3-mof interaction is detected only in the presence of msl-1.
Source was cell extract of baculovirus-infected Sf9 cell line; bait produced from transfected construct; prey produced from transfected construct.
FBrf0191180-17.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mof 
MOF
bait
TAP tag
MSL-3
prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
Source was cell extract of Sg4 cell line; bait produced from transfected construct; prey produced from endogenous gene.
FBrf0191180-30.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mof 
MOF
bait
TAP tag
MSL-3
prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
FBrf0191180-4.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mof 
MOF
bait
TAP tag
MSL-3
prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
Positive control.
Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
FBrf0191180-40.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
MSL-3
bait
FLAG tag
mof 
MOF
prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
FBrf0191180-53.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mof 
MOF
bait
MSL-3
prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
FBrf0191180-72.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mof 
MOF
bait
MSL-3
prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
FBrf0217803-2.AF.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mof 
mof
neutral component
mof 
mof
neutral component
mof 
mof 
mof
neutral component
mof 
msl-3
neutral component
msl-3
neutral component
msl-3
neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mof 
interaction domain
sufficient binding region
aa 350-827
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
Interaction in vitro; proteins produced as a recombinant fusion proteins in a baculovirus and insect cell system.
mof, msl-1 and msl-3 form a trimeric complex, captured as pairwise interactions.
FBrf0222216-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Msl3
bait
FLAG tag
mof 
Mof
prey
HA tag
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
Positive control.
Proteins were expressed individually in Sf21 cells and whole cell extracts were mixed.
Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-infected Sf21 cell system; prey produced as recombinant fusion protein in baculovirus-infected Sf21 cell system.
FBrf0225528-41.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
MSL1
bait
BioTAP tag
mof 
MOF
prey
26 unique peptides identified
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
Source was cross-linked nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Crude nuclear extracts were cross-linked using formaldehyde, sonicated, and subjected to ProteinA affinity purification with rabbit IgG agarose beads eluted under denaturing conditions, and subsequently using streptavidin agarose beads for Bio affinity purification. Purified peptides were eluted by on-bead trypsin digestion and mass spectrometric analysis.
Enrichment of prey protein was compared to input, mock and unrelated BioTAP affinity purifications using a statistical method (Bamse) developed by the authors to control for multiple sources of bias.
FBrf0225528-65.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
MSL3
bait
BioTAP tag
mof 
MOF
prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
Source was cross-linked nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Positive control.
One step, IgG-mediated pulldown of ProteinA portion of BioTAP tag.
FBrf0227292-420.TFIN
Description
physical association
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
unspecified role
unspecified role
unspecified role
mof 
unspecified role
mof 
unspecified role
mof 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
Source was cell extract of S2R+ cell line; proteins produced from transfected construct or endogenous gene.
HGScore = 14.41017
FBrf0229405-9.PLA
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
msl-3
neutral component
msl-3
neutral component
msl-3
neutral component
mof 
mof
neutral component
mof 
mof
neutral component
mof 
mof
neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
Factors were detected on polytene chromosomes using antibodies (to proteins) or biotinylated antisense probes (to ncRNA). The PLA signal develops only if probes to the two factors are in close proximity to each other (within tens of nanometers).
Source was larval salivary gland of wild-type fly line; proteins produced from endogenous gene.
FBrf0232931-3.coIP.MS
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
MSL3
bait
BioTAP tag
mof 
MOF
prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
FBrf0235529-5.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mof 
mof
bait
GFP tag
mof 
msl-3
prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mof 
interaction domain
mutation disrupting interaction
aa 532,539,541,545,694,706,715,773,776
K532R,K539R,K541R,K545R,K694R,K706R,K715R,K773R,K776R
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (10)