Transgenic flies carried a heat-inducible transgene encoding an activated tor-Egfr chimeric protein.

drk binds to activated, tyrosine-phosphorylated Egfr.

Source was adults of transgenic fly line; bait produced from endogenous gene; prey produced from transgenic construct.

Positive control.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type adult extract.

Source was adult female ovaries of wild-type or transgenic fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Overexpression of Cbl reduces the Egfr-drk interaction.

Two-hybrid system: yeast

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Overall SAINT probability of interaction = 1.