Interaction in vitro; proteins produced and purified from baculovirus-infected High Five cells.
Kd = 4.7E10-7 M
Interaction in vitro; bait derived from conditioned media of transfected S2 cell line; prey derived from conditioned media of transfected S2 cell line.
Interaction in vitro; bait derived from conditioned media of transfected S2 cell line; prey derived from conditioned media of transfected S2 cell line.
Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-infected High Five cell system; prey produced as a recombinant fusion protein in baculovirus-infected High Five cell system.
Kd = 0.37 uM
Interaction in vitro; proteins produced as a recombinant fusion protein in baculovirus-infected High Five cell system.
Interaction in vitro; bait derived from conditioned media of transfected S2 cell line; prey derived from conditioned media of transfected S2 cell line.
Interaction in vitro; bait derived from conditioned media of transfected S2 cell line; prey derived from conditioned media of transfected S2 cell line.
I83D coordinates relative to DIP-α-PA
H110K,V164K coordinates relative to dpr6-PC
Interaction in vitro; bait produced as a recombinant fusion protein in HEK293F cells; prey produced as a recombinant fusion protein in HEK293F cells.
Kd = 2.06uM
Interaction in vitro; bait purified from transfected HEK293F cells; prey purified from transfected HEK293F cells.
Kd = 2.0uM, average of 5 independent measurements
Source was live Sf9 insect cells; proteins produced from baculovirus constructs.
Physical interaction of proteins expressed on the surface of cells is inferred from the aggregation of live cells.
Assay: cell aggregation. Addition of phospholipase-C disrupts interaction.
