FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000098662
Interaction Network
Interactions Browser links
DIP-η network
dpr1 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0222867-128.ELISA
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

CG14010

bait

Fc tag

dpr1 

dpr

prey

alkaline phosphatase tag, COMP pentamerization motif

dpr1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
extracellular domain
sufficient binding region
dpr1 
extracellular domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from conditioned media of transfected S2 cell line; prey derived from conditioned media of transfected S2 cell line.

FBrf0222867-42.ELISA
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dpr1 

dpr

bait

Fc tag

dpr1 

CG14010

prey

alkaline phosphatase tag, COMP pentamerization motif

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dpr1 
extracellular domain
sufficient binding region
extracellular domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from conditioned media of transfected S2 cell line; prey derived from conditioned media of transfected S2 cell line.

FBrf0230454-66.ELISA
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

DIP-η

bait

Fc tag

dpr1 

dpr1

prey

alkaline phosphatase tag, COMP pentamerization motif

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from conditioned media of transfected S2 cell line; prey derived from conditioned media of transfected S2 cell line.

FBrf0230454-9.ELISA
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dpr1 

dpr1

bait

Fc tag

DIP-η

prey

alkaline phosphatase tag, COMP pentamerization motif

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from conditioned media of transfected S2 cell line; prey derived from conditioned media of transfected S2 cell line.

FBrf0241075-41.SPR
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

DIP-η

bait
dpr1 

Dpr1

prey
dpr1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
extracellular domain
sufficient binding region
aa 31-338

coordinates relative to DIP-η-PB

dpr1 
extracellular domain
sufficient binding region
aa 30-261

coordinates relative to dpr1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in HEK293F cells; prey produced as a recombinant fusion protein in HEK293F cells.

FBrf0241500-1.XC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dpr1 

Dpr1

neutral component
dpr1 

Dpr1

neutral component
dpr1 
dpr1 

Dpr1

neutral component
dpr1 

DIP-η

neutral component

DIP-η

neutral component

DIP-η

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dpr1 
IG1 domain
sufficient binding region
unspecified
IG1 domain
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in baculoviral Sf9 cell system.

FBrf0241500-3.SPR
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

DIP-η

bait
dpr1 

Dpr1

prey
dpr1 
dpr1 
dpr1 
dpr1 
dpr1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dpr1 
IG1 domain
sufficient binding region
unspecified
IG1 domain
sufficient binding region
unspecified
dpr1 
IG1 domain residue 94
mutation increasing interaction
aa 94

H94A

dpr1 
IG1 domain residue 95
mutation decreasing interaction
aa 95

I95A

dpr1 
IG1 domain residue 103
mutation decreasing interaction
aa 103

Y103A

dpr1 
IG1 domain residue 138
mutation decreasing interaction
aa 138

Q138A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion proteins in baculoviral Sf9 cell system; prey produced as a recombinant fusion proteins in baculoviral Sf9 cell system.

FBrf0241500-4.SPR
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dpr1 

Dpr1

bait
dpr1 

DIP-η

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dpr1 
IG1 domain
sufficient binding region
unspecified
IG1 domain
sufficient binding region
unspecified
IG1 domain residue 83
mutation decreasing interaction
aa 83

T83A

IG1 domain residue 84
mutation decreasing interaction
aa 84

I84A

IG1 domain residue 92
mutation decreasing interaction
aa 92

I92A

IG1 domain residue 126
mutation decreasing interaction
aa 126

Q126A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion proteins in baculoviral Sf9 cell system; prey produced as a recombinant fusion proteins in baculoviral Sf9 cell system.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)