Open Close
General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000098773
Interaction Network
Interactions Browser links
X11L network
rst network
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0190176-1.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
rst 

rst

bait

fused to LexA DNA-binding domain

rst 
rst 
X11L 

x11Lα

prey

fused to B42 activation domain

X11L 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
rst 
intracellular domain
sufficient binding region
aa 558-764
rst 
PDZ binding domain
necessary binding region
THV
X11L 
PDZ domain
sufficient binding region
aa 964-1168
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/B42-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

Nine rst-interacting clones corresponded to X11L.

FBrf0190176-4.coIP.WB
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
X11L 

X11Lα

bait

Myc tag

rst 

Rst

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

FBrf0190176-5.coIP.WB
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
rst 

Rst

bait
rst 
X11L 

X11Lα

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
rst 
intracellular domain
necessary binding region

rst[CT] mutant

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged endogenous gene; prey produced from endogenous gene.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)