FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ex, Mer
FlyBase ID
FBig0000098814
Interaction Network
Interactions Browser links
ex network
Mer network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0125175-1.FW.TV
Reference
McCartney et al., 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mer 

MER

bait

GST tag

Mer 
ex 

EX

prey

GST tag, biotin label

ex 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mer 
C-terminal region
sufficient binding region
aa 522-635
ex 
CNTR domain
sufficient binding region
aa 1-399
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(McCartney et al., 2000)

The ex CNTR domain binds to Mer but not Moe.

(McCartney et al., 2000)

Bait protein was separated by SDS-PAGE and transferred to nitrocellulose, then incubated with biotinylated prey protein.

(McCartney et al., 2000)
FBrf0247729-3.coIP.WB
Reference
Yang and Choi, 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
ex 

Ex

bait

V5 tag

Mer 

Mer

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Yang and Choi, 2021)
FBrf0259436-4.coIP.WB
Reference
Li et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mer 

Mer

bait

Fg tag

ex 

ex

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK-293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Li et al., 2024)

Addition of TER94 decreases Mer-ex interaction. Addition of ubiquitin increases Mer-ex interaction.

(Li et al., 2024)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)