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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000098940
Interaction Network
Interactions Browser links
AGO1 network
mir-13b-1 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0191204-1.coIP.NB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO1 

AGO1

bait

HA tag

miR-13b

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

mir-13b-1 (mature miRNA and pre-miRNA) coimmunoprecipitates with AGO1, and to a lesser extent, with AGO2 as well.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0204460-3.coIP.NB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO1 

AGO1

bait

HA tag

AGO1 

miR-13b

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
AGO1 
central region
mutation decreasing interaction
aa 594-629

F594V, F629V \'F2V2\' mutant, coordinates relative to 984 residue isoform

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0220359-5.coIP.NB
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO1 

Ago1

bait

FLAG tag

AGO1 

miR-13b

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
AGO1 
MID domain
mutation disrupting interaction
aa 657-694

K657A, K694A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

Embryo extracts were crosslinked, immunoprecipitated and bound miRNA assayed by Northern blot.

Positive control.

External Crossreferences and Linkouts ( 0 )
References (3)