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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000098963
Interaction Network
Interactions Browser links
Spt5 network
pho network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0222269-1.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Spt5 

Spt5

bait

fused to GAL4 DNA-binding domain

Spt5 
pho 

Pho

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Spt5 
Developmental Domain (DD)
sufficient binding region
aa 926-1078
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from normalized cDNA expression library).

Three independent Spt5-interacting cDNAs corresponded to pho.

FBrf0222269-2.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Spt5 

Spt5

bait

GST tag

Spt5 
pho 

Pho

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Spt5 
Developmental Domain (DD)
sufficient binding region
aa 926-1078
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

Interaction of pho with the Spt5 DD domain is weak but specific.

The G994D mutation of Spt5W049 does not perturb the Spt5-pho interaction.

FBrf0222269-4.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Spt5 

Spt5

bait

FLAG tag

pho 

Pho

prey

Myc tag

pho 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pho 
N-terminal region
sufficient binding region
aa 1-351
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

The G994D mutation of Spt5W049 does not perturb the Spt5-pho interaction.

FBrf0237429-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Spt5 

Spt5

bait

FLAG tag

pho 

Pho

prey

HA tag

pho 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pho 
REPO domain
mutation decreasing interaction
aa 148-169aa 164

V164D

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

The Spt5-pho interaction increases upon heat shock and presumable Hsf recruitment to chromatin. Increase in the Spt5-pho interaction upon heat shock requires a wildtype Hsf background, is unaffected by the V164D pho mutation, and is dependent on RNA.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)