Open Close
General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000099002
Interaction Network
Interactions Browser links
hkb network
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0222788-15.PD.TV
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hkb 

HKB

bait

MBP tag

hkb 

HKB

prey

luciferase tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

From an analysis of many TF-ChIP data sets, TF-TF interactions were predicted on the basis of regular DNA sequence motif arrangements. 27 predicted TF-TF interactions were tested using a pull down assay that measures the \'Luminescence Intensity Ratio\'. A cut-off of 7 was used, well above values for six negative controls.

Interaction in vitro; bait produced by coupled in vitro translation; prey produced by coupled in vitro translation.

FBrf0222788-20.PD.TV
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hkb 

HKB

self

luciferase tag

hkb 

HKB

self

luciferase tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

hkb protein was incubated with biotin labeled DNA containing two copies of its cognate DNA binding motif. DNA was pulled down with streptavidin beads and the amount of luciferase activity pulled down was measured. Unlabeled competitor oligos lacking one or both DNA binding motifs were added in excess to assess the cooperativity of DNA binding by the TF.

Unlabeled competitors containing only one motif were far less effective at competing for binding than oligos containing two nearby motifs. Binding of hkb to its cognate motif is cooperative.

Interaction in vitro; protein produced by in vitro translation.

External Crossreferences and Linkouts ( 0 )
References (1)