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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000099198
Interaction Network
Interactions Browser links
Arl1 network
Golgin245 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0206354-17.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Arl1 

Arl1

bait

GST tag

dGolgin-245

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The GTP-bound form of Arl1 binds CG3493, CG10703 and cbs.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

FBrf0206354-9.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Arl1 

Arl1

bait

fused to GAL4 DNA-binding domain

dGolgin245

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
C-terminal region
sufficient binding region
aa 1300-1489
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

From a panel of 27 small G proteins tested, CG3493 interacted with Arl1, Rab2 and Rab30.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

FBrf0217370-28.PD.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Arl1 

Arl1

bait

His tag

golgin-245

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

Golgin245 interacts preferentially with a version of Arl1 that mimics the GTP-bound state, over one that mimics the GDP-bound state.

The N-terminal amphipathic helix of Arf protein was replaced with 10-His residues. His-tagged bait Arf protein was then used to coat the surface of liposomes containing low levels of a lipid with Ni-nitrilotriacetic acid (NTA) head group. Bait-coated liposomes are mixed with cell lysate, then liposomes are purified by centrifugation. This allows for the identification of protein interactions that occur preferentially or exclusively at the surface of a lipid bilayer.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)