wts, Mer Physical Interaction report

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Mer

bait

HA tag

Mer

wts

Wts

prey

V5 tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Mer

FERM domain

sufficient binding region

aa 1-375

Mer

C-terminal region

mutation increasing interaction

aa 601-635

ΔC-terminus

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Yin et al., 2013)

wts coimmunoprecipitates with Mer but not ex in this assay.

(Yin et al., 2013)

The C-terminus of Mer inhibits the interaction with wts and presumably regulates Mer activity.

(Yin et al., 2013)

Mer promotes membrane association of wts.

(Yin et al., 2013)

The interaction of wts with full length Mer is enhanced by treatment with drugs that disrupt the actin cytoskeleton (C3, Latrunculin B).

(Yin et al., 2013)

FBrf0222652-5.coIP.WB

Reference

Yin et al., 2013

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Mer

bait

wts

Wts

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from endogeous gene; prey produced from endogeous gene.

(Yin et al., 2013)

FBrf0222652-7.coIP.WB

Reference

Yin et al., 2013

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

wts

Wts

bait

V5 tag

Mer

prey

HA tag

Mer

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Mer

FERM domain

sufficient binding region

aa 1-375

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Yin et al., 2013)

wts coimmunoprecipitates the N-terminal half of Mer, but not full length Mer.

(Yin et al., 2013)

FBrf0222652-8a.coIP.WB

Reference

Yin et al., 2013

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Mer

bait

HA tag

Mer

wts

Wts

prey

GFP tag

wts

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Mer

FERM domain

sufficient binding region

aa 1-375

wts

N-terminal region

sufficient binding region

aa 1-50

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Yin et al., 2013)

The interaction of wts with the Mer FERM domain is inhibited by co-transfection of a separate construct bearing the Mer C-terminus (aa 375-635).

(Yin et al., 2013)

FBrf0259436-3.coIP.WB

Reference

Li et al., 2024

Description

physical association

Assay