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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000099365
Interaction Network
Interactions Browser links
Kr network
ovo network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0222788-17.PD.TV
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Kr 

KR

neutral component

MBP tag

Kr 

KR

neutral component

MBP tag

Kr 

KR

neutral component

MBP tag

ovo 

OVO

neutral component

luciferase tag

ovo 

OVO

neutral component

luciferase tag

ovo 

OVO

neutral component

luciferase tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

ovo and Kr proteins were incubated with biotin labeled DNA containing both cognate DNA binding motifs. DNA was pulled down with streptavidin beads and the amount of luciferase activity pulled down was measured. Unlabeled competitor oligos lacking one or both DNA binding motifs were added in excess to assess the cooperativity of DNA binding by the two TFs.

Unlabeled competitors containing both ovo and Kr DNA motifs within the same oligo efficiently compete binding. When ovo and Kr motifs are provided on separate DNA molecules, they are not able to compete. This suggests that binding of ovo and Kr to oligos containing both cognate sites is cooperative.

Interaction in vitro; proteins produced by coupled in vitro translation; prey produced by coupled in vitro translation.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)