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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000100092
Interaction Network
Interactions Browser links
Leash network
yki network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0223213-20.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
leash
bait
HA tag
yki 
yki
prey
Myc tag
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ubiquitinated lysine residues
mutation decreasing interaction
aa 74-148
K74R, K116R and K148R: \'3K/R\'
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.
FBrf0223213-24.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
yki 
yki
bait
Myc tag
Leash
prey
HA tag
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Arrestin domains
sufficient binding region
aa 1-305
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.
FBrf0224345-189.coIP.MS
Description
physical association
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
yki 
yki
bait
Protein A tag, Strep tag
CG4674
prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.
FBrf0224619-25.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Leash
bait
HA tag
yki 
yki
prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.
Top 50 prey proteins from Leash AP/MS are reported in supplementary table S8. Only the validated yki-Leash interaction was curated for FlyBase.
FBrf0238240-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
yki 
Yki
bait
Myc tag
Leash
prey
HA tag
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)