FB2025_02 , released April 17, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Khc-73, 14-3-3ζ
FlyBase ID
FBig0000100643
Interaction Network
Interactions Browser links
Khc-73 network
14-3-3ζ network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0223553-2.PD.WB
Reference
Lu and Prehoda, 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Khc-73 

Khc73

bait

GST tag

Khc-73 
Khc-73 
14-3-3ζ 

14-3-3ζ

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Khc-73 
stalk region
sufficient binding region
aa 1336-1447

coordinates relative to Khc-73-PF

Khc-73 
14-3-3 binding motif
mutation increasing interaction
aa 1374

S1374D, phosphomimetic, coordinates relative to Khc-73-PF

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Lu and Prehoda, 2013)

Khc-73 binds to 14-3-3ζ but not 14-3-3ε.

(Lu and Prehoda, 2013)
FBrf0223553-7.coIP.WB
Reference
Lu and Prehoda, 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Khc-73 

Khc73

bait

Myc tag

Khc-73 
14-3-3ζ 

14-3-3ζ

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Khc-73 
14-3-3 binding motif
mutation disrupting interaction
aa 1374

S1374A, blocks phosphorylation, coordinates relative to Khc-73-PF

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK cell line; bait produced from transfected construct; prey produced from transfected construct.

(Lu and Prehoda, 2013)

Khc-73, nudE, 14-3-3ζ and 14-3-3ε form a quaternary complex, with a 14-3-3 heterodimer bridging Khc-73 and nudE.

(Lu and Prehoda, 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)