FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
usp, Utx
FlyBase ID
FBig0000100822
Interaction Network
Interactions Browser links
usp network
Utx network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0223700-2.PD.A
Reference
Denton et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Utx 

dUTX

bait

GST tag

Utx 
usp 

Usp

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Utx 
C-terminal region
sufficient binding region
aa 820-1136
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Denton et al., 2013)

Utx binds EcR/usp in both the presence and absence of ecdysone.

(Denton et al., 2013)

GST-Utx was mixed with both EcR and usp simultaneously for the pull down.

(Denton et al., 2013)
FBrf0229176-25a.coIP.MS
Reference
Xie et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / prepupal stage P1
Cell line used
Participants
Corresponds to
Reported as
Role
Note
usp 

USP

bait
Utx 

Utx

prey

1 peptide identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was white prepupae of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Xie et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)