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FB2025_05
,
released December 11, 2025
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transgenic adult extract.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system (prey from embryo cDNA expression library).
Two of 28 positive clones (from 500,000 plaques screened) corresponded to Sos.
Bait GST-drk protein was [32]P labeled in vitro at an engineered PKA site in the linker, and used to probe a λgt11 cDNA expression library.
Positive control.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Overall SAINT probability of interaction = 1.
Interaction in vitro; drk protein produced as a recombinant fusion protein in bacterial system; Sos peptide chemically synthesized
Interaction in vitro; SOS peptide chemically synthesized; drk protein produced as a recombinant fusion protein in bacterial system.
IDR sites 1,3 and 4 contain mutations to focus binding on site 2.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
IDR sites 1,3 and 4 contain mutations to focus binding on site 2.
Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system; RNA chemically synthesized.
aa 1339-1350; coordinates relative to Sos-PA
aa 1377-1388; coordinates relative to Sos-PA
Interaction in vitro; Drk protein produced as a recombinant fusion protein in bacterial system; Sos peptide chemically synthesized.