FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Raf, Dsor1
FlyBase ID
FBig0000101043
Interaction Network
Interactions Browser links
Raf network
Dsor1 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0105423-2.Y2H
Reference
Therrien et al., 1998
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Raf 

DRaf

bait

fused to LexA DNA-binding domain

Dsor1 

MEK

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Therrien et al., 1998)

Two-hybrid system: yeast LexA-BD/B42-AD, Dsor1-PB isoform was used.

(Therrien et al., 1998)
FBrf0224702-172.coIP.MS
Reference
Friedman et al., 2011
Description
physical association
Assay
Collection
RTK-Ras-ERK_Interactome
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Dsor1 

Dsor1

bait

TAP tag

Raf 

phl

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Friedman et al., 2011)

Overall SAINT probability of interaction = 0.93.

(Friedman et al., 2011)
FBrf0224702-300.coIP.MS
Reference
Friedman et al., 2011
Description
physical association
Assay
Collection
RTK-Ras-ERK_Interactome
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Raf 

phl

bait

TAP tag

Dsor1 

Dsor1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Friedman et al., 2011)

Overall SAINT probability of interaction = 0.93.

(Friedman et al., 2011)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)