FB2020_06, released Dec 22, 2020

Physical Interaction report

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General Information
Interaction Type
physical association
Interacting Genes
Ulp1, krz
FlyBase ID
FBig0000101112
Interaction Network
Interactions Browser links
Ulp1 network
krz network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0223495-1.coIP.MS
Reference
Anjum et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
krz 

Krz

bait

Protein A tag, Strep tag

Ulp1 

Ulp1

prey

23 unique peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Anjum et al., 2013)
FBrf0223495-2.coIP.WB
Reference
Anjum et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ulp1 

Ulp1

bait

V5 tag

Ulp1 
Ulp1 
krz 

Krz

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ulp1 
C-terminal region
sufficient binding region
aa 1286-1513
Ulp1 
central region
sufficient binding region
aa 647-1286

weak binding

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced by in vitro translation; prey produced by in vitro translation.

(Anjum et al., 2013)
FBrf0223495-4.coIP.WB
Reference
Anjum et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Ulp1 

Ulp1

bait

V5 tag

krz 

Krz

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Anjum et al., 2013)
FBrf0223495-5.coIP.WB
Reference
Anjum et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
krz 

Krz

bait

HA tag

Ulp1 

Ulp1

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Anjum et al., 2013)
FBrf0234792-4.coIP.WB
Reference
Zhang et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Ulp1 

Ulp1

bait

HA tag

krz 

Krz

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhang et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)