FB2025_01 , released February 20, 2025
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
Nap1, SMC1
FlyBase ID
FBig0000101948
Interaction Network
Interactions Browser links
Nap1 network
SMC1 network
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0222894-15.PD.A
Reference
Moshkin et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nap1 

NAP1

bait

HA tag

SMC1 

SMC1

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in Sf9 baculovirus system; prey produced and labeled by in vitro translation.

(Moshkin et al., 2013)
FBrf0222894-6.coIP.WB
Reference
Moshkin et al., 2013
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 15blastoderm stagegastrula stageembryonic stage 8embryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3extended germ band stageembryonic stage 4embryonic stage 2embryonic cycle 9late extended germ band stageembryonic stage 5embryonic stage 12embryonic stage 11embryonic stage 3embryonic cycle 6embryonic cycle 8embryonic cycle 5embryonic cycle 7embryonic stage 13embryonic cycle 4embryonic stage 14embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nap1 

NAP1

bait
SMC1 

SMC1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Moshkin et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)