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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000102158
Interaction Network
Interactions Browser links
Atg1 network
Atg13 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0207848-1.coIP.WB
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Atg1 

Atg1

bait

Myc tag

Atg1 

Atg13

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Atg1 
kinase domain
mutation increasing interaction
aa 38

K38Q

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval fat body of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

The Atg1-Atg13 interaction is increased in starved larvae. By contrast, a kinase-dead Atg1 (K38Q) interacts with Atg13 more strongly in fed larvae.

FBrf0224629-5.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Atg13

bait

HA tag, GFP tag

Atg1 

Atg1

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
C-terminal region
sufficient binding region
aa 393-523
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0224629-6.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Atg13

bait

FLAG tag

Atg1 

Atg1

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larvae of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

FBrf0234023-1.coIP.WB
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Atg1 

Atg1

bait

GFP tag

Atg13

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval fat bodies of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)