FB2025_02 , released April 17, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ac, Act42A
FlyBase ID
FBig0000102188
Interaction Network
Interactions Browser links
ac network
Act42A network
Reported Interactions
FBrf0223751-2.coIP.MS
Reference
Hsiao et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
ac 

Ac

bait

Myc tag

Act42A 

Actin

prey

12 unique peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Hsiao et al., 2014)

Act42A coimmunoprecipitates with ac in nuclear, but not cytosolic, extracts.

(Hsiao et al., 2014)
FBrf0223751-4.coIP.WB
Reference
Hsiao et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
ac 

Ac

bait

Myc tag

Act42A 

Actin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Hsiao et al., 2014)

Actin coimmunoprecipitates with ac in nuclear, but not cytosolic, extracts.

(Hsiao et al., 2014)
FBrf0223751-9.Y2H
Reference
Hsiao et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Act42A 

Actin42A

bait

fused to GAL4 DNA-binding domain

ac 

ac

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Hsiao et al., 2014)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Hsiao et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)